Type	Antibody.
Target	6xHis
Immunogen	HHHHHH conjugated with KLH
Host/Source	Rabbit.
Type	Polyclonal.
Format	Affinity purified.
Pack Size	1.0 mg
Preparation	Affinity purified
Buffer System	Phosphate Buffered Saline, pH 7.2. Preservative:- 0.1% sodium azide
State	Liquid
Storage	Store at 4ºC
Application	ELISA
Dilution	1:1000 ( 1 ug/ml)
Specificity	ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA(HHHHHH-BSA). A 1:50,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using ICL, Inc HRPO conjugated Goat Anti Rabbit IgG at 1:28,000 and TMB as the substrate. Appropriate specificity controls were run. 293T cells were transfected with three different constructs of differing sizes. Whole cell extracts were analyzed and the results compared with lysates from non-transfected cells. The anti 6HIS was an effective antibody at 1:1000 ( 1 ug/ml) dilution (
Manufacturer	Immunology Consultants Laboratory, Inc.