Type	Antibody
Target	Sox2
Immunogen	Recombinant human SOX2 (a.a. 135-317)
Host/Source	Goat
Type	Polyclonal
Format	Liquid
Pack Size	100 ug
Preparation	Affinity purired
Buffer System	Phosphate buffered saline with 5% trehlose
State	Liquid
Storage	Aliquot and store at -20ºC to -70ºC
IHC	5-10 ug/ml
Western Blot	5-10 ug/ml
ELISA	0.5 - 1.0 ug/ml
Storage	Store at -20ºC to -70ºC for 6 months without detectable loss of activity. The antibody can be stored at 2 - 8ºC for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Immuno- cytochemistry	This antibody has been used at 5-10ug/ml to detect human Sox2. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 0.1% Triton X-100, 1% BSA and 10% normal donkey serum at room temperature for 45 minutes. After blocking, sections were incubated with diluted primary antibody overnight at 4C followed by Rhodamine Red coupled anti-goat IgG antibody or another appropriate secondary antibody at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Wester Blot	This antibody has been used at 0.1 - 0.2 ug/ml with the appropriate secondary reagents to detect human Sox2. The detection limit for recombinant human SOX2 is approximately 2ng/lane under non-reductin conditions