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We offer a custom service for preparing, dispensing and stabilising antibody or antigen into 96-well microwell ELISA plates.
96 well microwell pates from a range of manufacturers are stocked as Strips or Solid plates or you can specify your preferred source.
There is no minimumn batch size. High performance automated coating is employed for 10 or more plates, due to the dead volume required by the dispenser. Small batches are processed using calibrated multichannel pipettes.
Standard Operating Procedures are followed, detailing coating, blocking and stabilising conditions. Full batch records are maintained for tracability, auditing and certification.
Summary
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Antibody or antigen is prepared in coating buffer
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Antibody or antigen is dispensed into the microwell plates.
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Plates are incubated to allow maximum coating
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Uncoated plate surfaces are blocked with a Block /Stabilising solution to prevent non-specific binding and protect the coating material during long term storage.
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Plates are vacuum dried and heat-sealed in zip-seal foil pouches containg silica gel to prevent degradation by moisture.
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Foil pouches are resealable after opening for extended use
Specifications
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ELISA plate formats - Stripwell or solid
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Blocking agent - Yes
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Protein Stabiliser - Yes
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Plate coating precision - <4%
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Presentiation - Heat-sealed, re-sealable foil pouches with dessicant
Advantages of custom coating
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Good precision
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No minimum batch size
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Long term stability
Related products: Contract ELISA Kit Production
Call [UK +44] (0)1803 526556 to discuss your requirements.
Strict confidentiallity is observed.
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